Repeated Cycles of amplification cause degradation of your probe, resulting in improved fluorescence. TaqMan detection is, therefore, far more distinct than dye‐dependent detection, because a rise in fluorescence takes place only on binding of your probe to a selected sequence and subsequent de‐quenching, even though no sign is made by https://www.facebook.com/NewTechFind/posts/pfbid028suFpZc3zrsMyMH2s35rRvKiiV5ZaLFRtKXBWSmig5vMs847oSeFLEF184pRjxtfl