In the present study. we attempted to control the pH profile of the catalytic activity of the industrially relevant alkaline protease KP-43. by incorporating 3-nitro-l-tyrosine and 3-chloro-l-tyrosine at and near the catalytic site. Thirty KP-43 variants containing these non-natural amino acids at the specific positions were synthesized in Escherichia coli host cells with expanded gen... https://www.spidertattooz.com/Interlude-Home-Nuage-Sofa-Down/