Extenders (3) were compared for frozen-thawed semen quality of Marwari horses in the present study. Ejaculates (36) including 6 ejaculates from each of 6 stallions were collected using artificial vagina (AV) method. The ejaculates were cryopreserved either with glucose-EDTA-lactose. HF-20 or egg yolk-skim milk extender using custom freezing technique. https://steedaes.shop/product-category/water-pump/